Description: Background: Acinetobacter baumannii (AB) is critical for healthcare-associated infections (HAI) with significant regional differences in the resistance rate, but its risk factors and infection trends have not well studied. Carbon nanotubes (CNTs) are substantially cylindrical molecules made entirely of carbon atoms and can use as nanocarriers. Multi-wall carbon nanotubes (MWCNTs) through their unique properties hold great promise in the fight against multidrug-resistant bacterial infections. Aim: In this study, Antimicrobial effects study of carbon nanotubes on Acinetobacter baumannii to prevent nosocomial infections done. Methods: Multi-wall carbon nanotubes provided from US Research and Cell viability assay were carried out after incubation of Acinetobacter baumannii with the CNTs suspensions (100 µg ml-1) for 24 h by Microplate Alamar Blue Assay (MABA) method. Results: Antimicrobial potential of carbon nanotubes on Acinetobacter baumannii was found based on the color change associated with the specified concentration range on the bacterial growth rate. Conclusion: This study showed that carbon nanotubes could have antimicrobial effects on Acinetobacter baumannii although to get more accurate results, we are doing a more specific cellular and molecular investigation.